Targeted lipidomics platform
Despite the significant role of lipid metabolism in biomedical research and the high potential in clinical application, effective monitoring of dynamic remodelling of the natural lipidome is difficult. On the one hand, this is due to the structural complexity of the lipids and, on the other hand, due to the large dynamic range of endogenous lipid concentrations. Modern bioanalytical methods based on mass spectrometry (MS) are able to resolve lipidome complexity but requires tailored solutions aiming either in-depth lipidome profiling or high-throughput screening. The goal of the project was to extend the existing MS technology platform to include an LC-MS/MS-based application for high-throughput quantitative analysis for phenotyping lipid metabolism. New QqQ instrument was integrated into the existing lipidomics infrastructure at ZML which allows now streamlined combination of untargeted and targeted lipidomics workflows under the same data analysis pipeline. New instrumentation was integrated into the Dresden Technology Portal.
Understanding dynamics of lipid metabolism and oxidation in ferroptotic cell death programme
Ferroptosis as a common underlying pathomechanism in tissue ischemia/reperfusion injury
Dysregulated systemic release of metabolic and bioactive lipids along hepatocyte-VLDL axis
Extension of the Lipidomics technology platform to include targeted high-throughput phenotyping of lipid metabolism and its remodelling